Pichia pastoris

High-level expression of Rhodotorula gracilis d -amino acid oxidase in Pichia pastoris

Engineering / Technology / Biotechnology / Biological Sciences / Propanol / Pichia pastoris / Enzyme / Industrial Application / Amino Acid Profile / Dry Matter / Pichia pastoris / Enzyme / Industrial Application / Amino Acid Profile / Dry Matter

Expression of a synthetic gene encoding a Tribolium castaneum carboxylesterase in Pichia pastoris

Escherichia coli / Protein Secretion / Animals / Pichia pastoris / Molecular cloning / Protein expression and purification / Tribolium / Specific Activity / Base Sequence / Heterologous Expression / Tribolium Castaneum / Biochemistry and cell biology / Protein expression and purification / Tribolium / Specific Activity / Base Sequence / Heterologous Expression / Tribolium Castaneum / Biochemistry and cell biology

Production of ferulic acid from lignocellulolytic agricultural biomass by Thermobifida fusca thermostable esterase produced in Yarrowia lipolytica transformant

Biomass / Multidisciplinary / Actinobacteria / Polymerase Chain Reaction / Lignin / Pichia pastoris / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Fermentation / Esterases / Base Sequence / Rice Bran / Yarrowia / Hydrolysis / Ferulic Acid / Wheat Bran / Pichia pastoris / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Fermentation / Esterases / Base Sequence / Rice Bran / Yarrowia / Hydrolysis / Ferulic Acid / Wheat Bran

Expresión de anticuerpos de cadena sencilla (ScFv) contra

Helicobacter pylori / Pichia pastoris

Functional characterisation of a putative rhamnogalacturonan II specific xylosyltransferase

NMR Spectroscopy / Arabidopsis thaliana / Phylogeny / Pichia pastoris / Arabidopsis / Molecular cloning / Substrate Specificity / Pectins / Recombinant Protein / Biochemistry and cell biology / Molecular cloning / Substrate Specificity / Pectins / Recombinant Protein / Biochemistry and cell biology

Viral Preprotoxin Signal Sequence Allows Efficient Secretion of Green Fluorescent Protein by Candida glabrata, Pichia pastoris, Saccharomyces cerevisiae, and Schizosaccharomyces pombe

Fluorescence Microscopy / Cell Biology / Multidisciplinary / Saccharomyces cerevisiae / Secretory Pathway / Mycotoxins / Protein Secretion / Applied Environmental Microbiology / Pichia pastoris / Green Fluorescent Protein / Schizosaccharomyces Pombe / Candida Glabrata / Yeasts / Gel electrophoresis / Biological activity / Culture Media / Recombinant Protein / Polyacrylamide Gel Electrophoresis / Recombinant Proteins / Full Length Movies / Mycotoxins / Protein Secretion / Applied Environmental Microbiology / Pichia pastoris / Green Fluorescent Protein / Schizosaccharomyces Pombe / Candida Glabrata / Yeasts / Gel electrophoresis / Biological activity / Culture Media / Recombinant Protein / Polyacrylamide Gel Electrophoresis / Recombinant Proteins / Full Length Movies

Comparative characterisation of recombinant invertebrate and vertebrate peptide O-Xylosyltransferases

Caenorhabditis elegans / Mass Spectrometry / Medical Microbiology / Drosophila melanogaster / Humans / Animals / Pichia pastoris / Glycosaminoglycan / Enzyme / High Pressure Liquid Chromatography / Liquid Chromatography / Electrospray Ionization Mass Spectrometry / Substrate Specificity / Enzyme activity / Heparan Sulphate / Species Specificity / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Neurosciences / Biochemistry and cell biology / Animals / Pichia pastoris / Glycosaminoglycan / Enzyme / High Pressure Liquid Chromatography / Liquid Chromatography / Electrospray Ionization Mass Spectrometry / Substrate Specificity / Enzyme activity / Heparan Sulphate / Species Specificity / Amino Acid Sequence / Base Sequence / Recombinant Proteins / Neurosciences / Biochemistry and cell biology

Functional characterisation of a putative rhamnogalacturonan II specific xylosyltransferase

NMR Spectroscopy / Arabidopsis thaliana / Phylogeny / Pichia pastoris / Arabidopsis / Molecular cloning / Substrate Specificity / Pectins / Recombinant Protein / Biochemistry and cell biology / Molecular cloning / Substrate Specificity / Pectins / Recombinant Protein / Biochemistry and cell biology

Functional characterisation of a putative rhamnogalacturonan II specific xylosyltransferase

NMR Spectroscopy / Arabidopsis thaliana / Phylogeny / Pichia pastoris / Arabidopsis / Molecular cloning / Substrate Specificity / Pectins / Recombinant Protein / Biochemistry and cell biology / Molecular cloning / Substrate Specificity / Pectins / Recombinant Protein / Biochemistry and cell biology

Study of a sterol esterase secreted by Ophiostoma piceae: Sequence, model and biochemical properties

Biological Sciences / Crystal structure / Physical sciences / Pichia pastoris / Enzyme / Molecular cloning / Substrate Specificity / Amino Acid Profile / Amino Acid Sequence / Binding Site / Molecular cloning / Substrate Specificity / Amino Acid Profile / Amino Acid Sequence / Binding Site

Expression of xyloglucan endotransglycosylases of Gerbera hybrida and Betula pendula in Pichia pastoris

Engineering / Technology / Biotechnology / Biomass / Culture / Filtration / Gene expression / Biological Sciences / Bioreactor / Methanol / Temperature / Pichia pastoris / Asteraceae / Enzyme / Betula / Specific Activity / Bioreactors / Glycosyltransferases / Amino Acid Profile / Molecular Mass / Plant Cell Wall / Amino Acid Sequence / Culture Media / Heterologous Expression / Betula Pendula / Minimal Medium / Cell Growth / Thallophyta / Filtration / Gene expression / Biological Sciences / Bioreactor / Methanol / Temperature / Pichia pastoris / Asteraceae / Enzyme / Betula / Specific Activity / Bioreactors / Glycosyltransferases / Amino Acid Profile / Molecular Mass / Plant Cell Wall / Amino Acid Sequence / Culture Media / Heterologous Expression / Betula Pendula / Minimal Medium / Cell Growth / Thallophyta

Characterization of a Gene Encoding a Pichia pastoris Protein Disulfide Isomerase

Protein Folding / Endoplasmic Reticulum / Pichia pastoris / Molecular cloning / Active site / Thioredoxin / Genomic DNA / Amino Acid Sequence / Base Sequence / Open Reading Frame / Full Length Movies / Biochemistry and cell biology / Molecular Sequence Data / Thioredoxin / Genomic DNA / Amino Acid Sequence / Base Sequence / Open Reading Frame / Full Length Movies / Biochemistry and cell biology / Molecular Sequence Data

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Pichia pastoris

High-level expression of Rhodotorula gracilis d-amino acid oxidase in Pichia pastoris

Engineering / Technology / Biotechnology / Stability / Biological Sciences / Propanol / Mechanism / Pichia pastoris / Enzyme / Industrial Application / Amino Acid Profile / Dry Matter / Propanol / Mechanism / Pichia pastoris / Enzyme / Industrial Application / Amino Acid Profile / Dry Matter

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